Cell membrane coated biomimetic nanoparticles (NPs) have been extensively studied in nanomedicine due to their distinctive properties reminiscent of immune escaping, lengthy blood circulation, particular molecular recognition and environment friendly most cancers concentrating on, indicating an excellent potential in focused most cancers remedy. However, the integrity of the cell membrane coating on NPs, a key metrics associated to the standard of those biomimetic-systems and to the ensuing biomedical perform, has remained largely unexplored. In the current research, the researchers developed a fluorescence quenching assay to probe the integrity of the cell membrane coating.
The research printed in Nature Communications reveals that the good majority of the cell membrane coated NPs have been solely partially coated when the normal coating methods have been utilized. The info is crucial because the coating diploma impacts the organic destiny of NPs. The analysis was carried out on the University of Eastern Finland, Department of Applied Physics, in Professor Vesa-Pekka Lehto’s analysis group.
“The present methods for characterizing the cell membrane coating are only qualitative and fail to statistically evaluate the degree and variability of the coating,” says Lizhi Liu, the primary creator of the publication. “When we applied the developed quantification method to evaluate the success of the commonly used protocols to produce fully coated NPs, we found that the fraction never exceeded 20 percent.”
“Our discovery is a big surprise to whole scientific community in nanomedicine because it has been generally accepted that the cell membrane coating is perfect. Despite of the partial coating, biomimetic NPs could still be internalized by the target cells via different pathways,” says Dr. Wujun Xu, one of many corresponding authors of the paper. To clarify this, the authors proposed a brand new endocytic entry mechanism for these partially coated NPs by computational simulations. Specifically, the NPs with a excessive coating diploma (≥ 50 %) entered the cells individually, whereas the NPs with a low coating diploma (
“The current study highlights among the limitations of the present cell membrane coating protocols and motivates the efforts to improve the protocols. The developed quantification method is a practical tool to assess the success of these efforts and establish a standard for comparing the different coating designs,” says Professor Vesa-Pekka Lehto.
Lizhi Liu et al, Cell membrane coating integrity impacts the internalization mechanism of biomimetic nanoparticles, Nature Communications (2021). DOI: 10.1038/s41467-021-26052-x
University of Eastern Finland
Novel fluorescence imaging assay provides new perception to develop biomimetic nanoparticles for focused most cancers remedy (2021, September 30)
retrieved 30 September 2021
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