Researchers reveal molecular mechanism of pannexin 2 as ATP membrane pore channel

A analysis workforce led by Prof. Yuan Shuguang from Shenzhen Institute of Advanced Technology of the Chinese Academy of Sciences and Prof. Zhang Huawei from the South University of Science and Technology has revealed the molecular mechanism of pannexin 2 (Panx2) as an ATP membrane pore channel.

The examine was revealed in Nature Communications on March 3.

ATP membrane pore channel protein performs an essential position in human physiological processes. Its abnormal function can result in extreme penalties corresponding to ischemic cerebral infarction, glioma, and pleomorphic malignant glioma.

The pannexins protein household, together with Panx1, Panx2, and Panx3, can type macroporous non-selective transmembrane (TM) channels. They are important in cell communication and homeostasis. The Panx2 protein is the most important pannexins member of the family, and it’s primarily expressed within the central nervous system.

Taking mind glioma for example, the total survival time of sufferers with a better degree of Panx2 is longer, which means that Panx2 might have an anti-tumor impact within the early stage of glioma.

The workforce solved the high-resolution construction of Panx2 by means of freezing electron microscope. They discovered that Panx2 is a four-TM area protein with seven monomer proteins gathering collectively, forming a transmembrane pore. By evaluating the buildings of Panx2 and Panx1, they speculated that Panx2 is perhaps the channel of ATP.

The workforce then verified the above speculation by means of ATP launch assay and molecular dynamics simulation. In the ATP launch assay, the effectivity of Panx2-NT-R89A was considerably increased than the counterpart of the wild-type Panx2. This outcome implied that amino acid R89 was chargeable for ATP passing by means of Panx2.

In addition, molecular dynamics simulation confirmed that the aspect chain of R89 swung flexibly, ensuing within the pore dimension improve of the channel accordingly. Such modifications corresponded to the diffusion of ATP.

“Our work illuminated the 3D buildings of Panx2 transmembrane protein on the atomic level,” mentioned Prof. Yuan. “It helps to understand the fundamentally biological function of Panx2 and provides an insightful view into related drug discovery as well.”