Scientists uncover the molecular mechanism of black-streaked dwarf virus in rice


RBSDV infection-induced autophagy in L. striatellus midguts at varied days post-feeding. Credit: Zhejiang University

Rice viruses are prevalent in lots of rice-growing international locations and infrequently trigger severe damages to rice manufacturing. Among them, the rice black-streaked dwarf virus (RBSDV), transmitted by the small brown planthopper Laodelphax striatellus, causes great losses in China’s grain yields yearly. Therefore, discovering the transmission mechanism of RBSDV is of immense significance for its efficient management.

The analysis staff led by Prof. Wu Jianxiang and Prof. Zhou Xueping from the Zhejiang University College of Agriculture and Biotechnology revealed an open-access article entitled “Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in L. striatellus” within the journal Autophagy.

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The analysis staff found that the early phase of RBSDV an infection in L. striatellus can induce autophagy, resulting in the suppression of RBSDV invasion and accumulation whereas inhibiting autophagy can promote RBSDV invasion and accumulation and thus enhance the mortality price of RBSDV-infected L. striatellus. This signifies that autophagy, as an innate immune response, performs an important function within the battle in opposition to RBSDV invasion.

Furthermore, the primary capsid protein (also called P10) of RBSDV alone can induce autophagy in each Sf9 and L. striatellus cells. Yeast two-hybrid (Y2H), pull down, Co-IP assays confirmed that RBSDV P10 can work together with GAPDH in vivo and in vitro. Further experiments indicated that Sf9 cells expressing RBSDV P10 can promote the phosphorylation of AMP-activated protein kinase (AMPK), leading to GAPDH phosphorylation and relocation of GAPDH from the cytoplasm to the nucleus.

Meanwhile, RBSDV invasion or feeding recombinant expressed RBSDV P10 also can promote LsAMPK phosphorylation, resulting in LsGAPDH phosphorylation and the translocation of the phosphorylated LsGAPDH from the cytoplasm to the nucleus to activate the autophagy pathway in L. striatellus. Co-IP and in vitro phosphorylation assays confirmed that AMPK interacts with GAPDH, phosphorylated AMPK can phosphorylate GAPDH, and silencing AMPK genes can inhibit the prevalence of GAPDH phosphorylation, translocation of GAPDH into the nucleus and autophagy.

This examine reveals that RBSDV invasion or RBSDV P10 can induce AMPK phosphorylation, which may result in GAPDH phosphorylation and the translocation of phosphorylated GAPDH into the nucleus. Once contained in the nucleus, phosphorylated GAPDH can activate autophagy to suppress virus an infection. “Our research illuminates the mechanism by which RBSDV induces autophagy in L. striatellus, and indicates that the autophagy pathway in an insect vector participates in the anti-RBSDV innate immune response,” mentioned Prof. Wu. “This will provide new insights into RBSDV control.”

Scientists discover the molecular mechanism of RBSDV inducing cell autophagy in Laodelphax striatellus
A working mannequin of RBSDV P10-induced autophagy in L. striatellus cells. Credit: Zhejiang University

Discovery of a mechanism for efficient autophagosome formation

More data:
Qi Wang et al, Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in Laodelphax striatellus, Autophagy (2021). DOI: 10.1080/15548627.2021.1954773

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Zhejiang University

Scientists uncover the molecular mechanism of black-streaked dwarf virus in rice (2021, September 6)
retrieved 6 September 2021

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