The research experiences that this assay relies on the usage of two isogenic HT1080 cell strains, one carrying a linear human synthetic chromosome (HAC) and the opposite carrying a round HAC.
‘Transient telomere dysfunction can induce chromosomal instability in human cells that may result in cancer. G4 ligands that induce telomere dysfunction and greatly increase chromosome mis-segregation rates are promising drug candidates for the treatment of cancer alone or in combination with ionizing radiation.’
G4 Ligand towards Cancer
It is understood that the most cancers drug therapy ends in particular destabilization of the linear HAC, as a result of disruption of telomeres. The research used the dual-HAC assay for the evaluation of the platinum-derived G4 ligand Pt-tpy and 5 of its derivatives.
The formation of G4s at telomeres might impede telomerase recognition and inhibit telomere elongation resulting in telomere shortening. Thus, telomeres are promising targets for the invention of ligands that stabilize G4s at telomeres, thereby perturbing telomere upkeep and resulting in genomic instability.
The research thereby means that G4 ligands that induce telomere dysfunction and drastically improve chromosome mis-segregation charges are promising drug candidates for the therapy of most cancers alone or together with ionizing radiation.